Akshata I.
Private tutor in San Diego, CA
Education
Master of Science (Biochemistry and Molecular Biology) Aug 2012 — Jun 2014 University Of Southern California, Los Angeles Master of Science (Medical Biochemistry) Aug 2010 —Jun 2012 Mumbai University, India Bachelor of Science (Biochemistry) Jun 2007 —Jun 2010 Mumbai University, India
Experience
Scientist Pfizer,Department of Vaccine Immunotherapeutics La Jolla,CA . February 2015-Present Developing assays like MDSC(myeloid derive suppressor cell),ICP(immune cell profiling),ICS(immune cytokine staining) using multicolor(13 to 15 color) flow cytometry. Daily maintainence of LSR Fortessa instrument,running application settings for the above described assays, use of compensation beads for compensation for each of the panels. Maintaining documentation pertaining to the daily performance like CS&T performance check and baseline of the flow cytometer. Isolating PBMC from human whole blood Working in GCLP environment on phase 1 clinical trial Recording data with use of Biobook(electronic laboaratory notebook),data presentation. Data analysis using Flow jo version 9.8 and graphpad prism. Research Technician II Dr. David Agus lab, Center for Applied Molecular Medicine University of Southern California, Los Angeles, CA July 2014-February 2015 Determining the role of AGR2 chaperone in metastasis and proliferation of cancer cells - Cell cycle analysis, cell sorting using flow cytometry - Generation of library using restriction enzymes adding adapters and ion torrent sequencing - Mammalian cell culture, tissue culture, transfection, viral transduction - DNA cloning, amplification, RT-PCR and site directed mutagenesis - Drug development and testing in mammalian tissue culture - Cell viability and cell proliferation assays - Maintaining lab inventory and Data Logs - Microbial, Biochemical and Hematological test execution - Data entry, maintaining spreadsheets and statistical analysis of samples - Data inventory, ordering lab supplies and maintaining logbooks Research Staff Dr. Preet Chaudhary Lab, Department of Hematology Norris Comprehensive Cancer Center, Los Angeles, CA December 2012- July 2014 -Construction of sh-RNA to knockout signaling proteins in PEL cell lines -Translational use of chimeric antigenic receptor CAR against CD19 -Studying the interaction of viral protein in progression of lymphoma -DNA cloning, siRNA and shRNA designing, RT-PCR -Western blot, immunoprecipitation, co-IP, Rodent Surgery and immuno histochemical staining of tissue samples, Flow cytometry, spectrophotometry -Generation of lentivirus, infection of mammalian cell line for establishment and maintenance of stable cell lines and maintaining Aseptic technique -Assisted in xenografting, detection of tumor development by flow sorting. -Maintaining mice colonies, breeding, weening and genotyping mice
Availability
Any day after 6PM, Weekends at any time
Can Meet
Up to 25 minutes away for no additional charge
Hobbies
Research,Teaching,Travelling,Driving,Cooking and mountaineering
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